Coding

Part:BBa_K163000

Designed by: Nora Yucel   Group: iGEM08_University_of_Chicago   (2008-08-22)

T4 phage gene T for self-lysis

Gene t encodes a holin, which is considered to degrade or alter the cytoplasmic membrane, thus allowing lysozymes from T4 gene e to reach the periplasm and gain access to the peptidoglycan layer. Essential component of the cell-lysis system triggered by T4 bacteriophage in E. coli. Expression induces cell-lysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None